mouse treg isolation kit Search Results


cd4+cd25+ regulatory t cell isolation kit, mouse  (Miltenyi Biotec)
98
Miltenyi Biotec cd4+cd25+ regulatory t cell isolation kit, mouse
Cd4+Cd25+ Regulatory T Cell Isolation Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd4+cd25+ regulatory t cell isolation kit, mouse/product/Miltenyi Biotec
Average 98 stars, based on 1 article reviews
cd4+cd25+ regulatory t cell isolation kit, mouse - by Bioz Stars, 2026-03
98/100 stars
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mouse treg isolation kit ii  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc mouse treg isolation kit ii
RGC-32 is preferentially induced in Th17 cells and promotes their differentiation. (A) Quantitative RT-PCR for RGC-32 in <t>naive</t> <t>CD4+</t> T cells from spleens of WT mice stimulated for 48 h with anti-CD3 and anti-CD28 in the presence or absence of indicated cytokines and in (B) Th0, Th1, Th2, Th17, or <t>Treg</t> polarizing conditions (**p, 0.01; mean 6 SEM; n = 3–4 mice per group). (C) Representative histogram of RGC-32 intracellular staining in CD4+ T cells cultured under Th17 and Treg conditions. Gray histogram represents background staining in RGC-32−/− CD4+ T cells. (D and E) Naive CD4+ T cells were cultured for 72 h in Th17 conditions and intracellular expression of IL-17A was assessed by flow cytometry. A representative profile is shown. Plots were gated on viable singlet CD4+ events. (F) ELISA for IL-17A determined in supernatants. (G) Real-time PCR for IL-17A. (H) Quantitative RT-PCR for Th17 signature genes IL-17F, IL-21, IL-22, and IL-23R. (*p, 0.05, **p, 0.01, mean 6 SEM). Data are representative of more than three independent experiments (n = 3–4 mice per group).
Mouse Treg Isolation Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse treg isolation kit ii/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
mouse treg isolation kit ii - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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RGC-32 is preferentially induced in Th17 cells and promotes their differentiation. (A) Quantitative RT-PCR for RGC-32 in naive CD4+ T cells from spleens of WT mice stimulated for 48 h with anti-CD3 and anti-CD28 in the presence or absence of indicated cytokines and in (B) Th0, Th1, Th2, Th17, or Treg polarizing conditions (**p, 0.01; mean 6 SEM; n = 3–4 mice per group). (C) Representative histogram of RGC-32 intracellular staining in CD4+ T cells cultured under Th17 and Treg conditions. Gray histogram represents background staining in RGC-32−/− CD4+ T cells. (D and E) Naive CD4+ T cells were cultured for 72 h in Th17 conditions and intracellular expression of IL-17A was assessed by flow cytometry. A representative profile is shown. Plots were gated on viable singlet CD4+ events. (F) ELISA for IL-17A determined in supernatants. (G) Real-time PCR for IL-17A. (H) Quantitative RT-PCR for Th17 signature genes IL-17F, IL-21, IL-22, and IL-23R. (*p, 0.05, **p, 0.01, mean 6 SEM). Data are representative of more than three independent experiments (n = 3–4 mice per group).

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: RGC-32 Promotes Th17 Cell Differentiation and Enhances Experimental Autoimmune Encephalomyelitis

doi: 10.4049/jimmunol.1602158

Figure Lengend Snippet: RGC-32 is preferentially induced in Th17 cells and promotes their differentiation. (A) Quantitative RT-PCR for RGC-32 in naive CD4+ T cells from spleens of WT mice stimulated for 48 h with anti-CD3 and anti-CD28 in the presence or absence of indicated cytokines and in (B) Th0, Th1, Th2, Th17, or Treg polarizing conditions (**p, 0.01; mean 6 SEM; n = 3–4 mice per group). (C) Representative histogram of RGC-32 intracellular staining in CD4+ T cells cultured under Th17 and Treg conditions. Gray histogram represents background staining in RGC-32−/− CD4+ T cells. (D and E) Naive CD4+ T cells were cultured for 72 h in Th17 conditions and intracellular expression of IL-17A was assessed by flow cytometry. A representative profile is shown. Plots were gated on viable singlet CD4+ events. (F) ELISA for IL-17A determined in supernatants. (G) Real-time PCR for IL-17A. (H) Quantitative RT-PCR for Th17 signature genes IL-17F, IL-21, IL-22, and IL-23R. (*p, 0.05, **p, 0.01, mean 6 SEM). Data are representative of more than three independent experiments (n = 3–4 mice per group).

Article Snippet: CD4 + CD25 + Tregs were purified using a mouse Treg isolation kit II (Stemcell Technologies).

Techniques: Quantitative RT-PCR, Staining, Cell Culture, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

RGC-32−/− CD4+ T cells polarize normally to Th1, Th2, and Treg lineages. Naive CD4+ T cells were skewed in vitro under Th1, Th2, and Treg conditions. (A) Intracellular expression of IFN-γ was assessed by flow cytometry. (B) ELISA for IFN-γ determined in supernatants. (C) Intracellular expression of IL-4 was assessed by flow cytometry. (D) ELISA for IL-4 determined in supernatants. (E) Foxp3 expression was determined by intracellular staining. (F) In vitro suppression assay of purified CD4+CD252 T responder cells cocultured for 3 d with in vitro–differentiated, purified CD4+CD25+ iTregs from WT or RGC-32−/− mice. Proliferation was assessed by [3H]thymidine incorporation added in the last 18 h of culture (left panel). Percentage inhibition at 1:1 and 1:2 Treg/T responder ratio is shown in the right panel. ***p, 0.001. Data represent the mean 6 SD and are representative of more than three independent experiments (n = 3 mice per group). Representative profiles shown for intracellular IL-4, IFN-γ, and Foxp3 are gated on viable singlet CD4+ events.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: RGC-32 Promotes Th17 Cell Differentiation and Enhances Experimental Autoimmune Encephalomyelitis

doi: 10.4049/jimmunol.1602158

Figure Lengend Snippet: RGC-32−/− CD4+ T cells polarize normally to Th1, Th2, and Treg lineages. Naive CD4+ T cells were skewed in vitro under Th1, Th2, and Treg conditions. (A) Intracellular expression of IFN-γ was assessed by flow cytometry. (B) ELISA for IFN-γ determined in supernatants. (C) Intracellular expression of IL-4 was assessed by flow cytometry. (D) ELISA for IL-4 determined in supernatants. (E) Foxp3 expression was determined by intracellular staining. (F) In vitro suppression assay of purified CD4+CD252 T responder cells cocultured for 3 d with in vitro–differentiated, purified CD4+CD25+ iTregs from WT or RGC-32−/− mice. Proliferation was assessed by [3H]thymidine incorporation added in the last 18 h of culture (left panel). Percentage inhibition at 1:1 and 1:2 Treg/T responder ratio is shown in the right panel. ***p, 0.001. Data represent the mean 6 SD and are representative of more than three independent experiments (n = 3 mice per group). Representative profiles shown for intracellular IL-4, IFN-γ, and Foxp3 are gated on viable singlet CD4+ events.

Article Snippet: CD4 + CD25 + Tregs were purified using a mouse Treg isolation kit II (Stemcell Technologies).

Techniques: In Vitro, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Staining, Suppression Assay, Purification, Inhibition